Identification of differentially expressed genes in two new human bladder carcinoma cell lines
- Author:
Yucong YANG
1
Author Information
1. Center for Experimental Medicine
- Publication Type:Journal Article
- Keywords:
Bladder cancer;
Cell lines;
Gene;
Suppression subtractive hybridization
- From:Academic Journal of Xi'an Jiaotong University
;19(2):182-185
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To screen and identify differentially expressed genes in two new human urothelial carcinoma cell lines, BLS-211 and BLX. Methods: Suppression subtractive hybridization (SSH) was used to createa subtracted library, and clones were sequenced. Results: Totally 13 over-expressed genes in BLX and 9 in BLS-211 cells were obtained, respectively. Among them, 18 were known genes and 4 were new ESTs (Expressed Sequence Tag), and were collected by GenBank dbEST database (The access number was EB390424-7). Conclusion: SSH is a powerful method for the identification of differentially expressed genes. The differential expression of some BCG-associated genes in different cells may be related to the different responses to clinical BCG therapy. The identified new ESTs can be cloned for full length to further study their functions.
