siRNA against survivin suppresses the proliferation of pancreatic cancer cell PC-2 and induces its apoptosis
- Author:
Haitao GUAN
1
Author Information
1. Department of Oncosurgery
- Publication Type:Journal Article
- From:Academic Journal of Xi'an Jiaotong University
2006;18(1):78-82
- CountryChina
- Language:Chinese
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Abstract:
Objective: Blocking the expression of Survivin with siRNA, and the effects of suppressing the proliferation of PC-2 cell and inducing its apoptosis were investigated. Methods: Constructed the siRNA against Survivin plasmid expression vector and transfected it into PC-2 cell with lipofectamine™ 2000, the changes of Survivin expression were detected by semi-quantitive RT-PCR and immunohistochemical method, The effect of suppressing the proliferation of PC-2 cell was detected by the method of MTT; the effect of inducing PC-2 cell apoptosis was detected by flow cytometry. Results: The sequence specific siRNA can effectively block the expression of Survivin both at the mRNA and protein levels, the expression inhibition ratio was 81.25% at mRNA level and 74.24% at protein levels blocking the expression of Survivin can suppress the proliferation of PC-2 cell significantly, 24, 48 hours after the cell was reseeded, the proliferation inhibition ratio was 28.00% and 33.38% respectively; 24, 48 hours after the transfection, 8.46% and 7.53% cells were induced to apoptosis respectively. Conclusion: The siRNA against Survivin plasmid expression vector constructed in the study can blocking the expression of Survivin in PC-2 cell effectively and specifically; blocking the expression of Survivin can significantly suppress the proliferation of PC-2 cell and induce a certain degree cells apoptosis; RNAi against Survivin is of a certain value in the gene therapy of pancreatic cancer.
