Detecting low density lipoprotein receptor mutant gene of rabbit by PCR
- Author:
Enqi LIU
1
Author Information
1. Laboratory Animal Center
- Publication Type:Journal Article
- From:Academic Journal of Xi'an Jiaotong University
2006;18(2):199-201
- CountryChina
- Language:Chinese
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Abstract:
Objective: Watanabe Heritable Hyperlipidaemic (WHHL) rabbits with low density lipoprotein receptor (LDLr) gene mutation have provided unprecedented opportunities for the study of human atherosclerosis, in order to confirm LDL receptor gene status in rabbits, we developed a simple PCR technique to detect LDL mutations in rabbits. Methods: Rabbits genomic DNA were extracted from ear biopsy, and amplified by PCR to detect 12 bp deletion mutation in WHHL rabbits. PCR products were directly digested with Bgl I, and then applied to polyacrylamide gel electrophoresis. Results: PCR products from homozygous LDLr +/+ rabbits generated 2 bands of 212 and 94 bp after Bgl I digestion, LDLr +/- rabbits generated 3 bands (294, 212, and 94 bp), LDLr -/-animals, however, generated only 1 product (294 bp). Conclusion: This modified PCR method is simple and reliable.
