Effect of Carrier on Labeling and Biodistribution of Re-188-Hydroxyethylidene diphosphonate.
- Author:
Young Soo CHANG
;
Jae Min JEONG
;
Bo Kwang KIM
;
Jung Hyuk CHO
;
Dong Soo LEE
;
June Key CHUNG
;
Seung Jin LEE
;
In Kul KIM
;
Sang Eun LEE
;
Myung Chul LEE
- Publication Type:Original Article
- Keywords:
Re-188-HEDP;
Carrier;
Labeling efficiency;
Stability
- MeSH:
Animals;
Etidronic Acid;
Hydrogen-Ion Concentration;
Kidney;
Mice;
Mice, Inbred ICR;
Rats;
Stomach
- From:Korean Journal of Nuclear Medicine
2000;34(4):344-352
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Re-188-Hydroxyethylidene diphosphonate (HEDP) is a new cost-effective agent for systemic radioisotope therapy of metastatic bone pain. We investigated the influence of carrier for labeling and biodistribution of Re-188-HEDP using HEDP kit with or without carrier (KReO4). MATERALS AND METHODS: The kits (HEDP 15 mg, gentisic acid 4 mg and SnCl2.2H2O 4.5 mg) with or without carrier (KReO4 0.1 mg) were labeled with Re-188 solution, made available from an in-house generator by boiling for 15 min. We compared the labeling efficiency and stability of carrier-added and carrier-free preparations of Re-188-HEDP. Biodistribution and imaging studies of each preparation were performed in ICR mice (1.85~3.7 MBq/0.1 ml) and SD rats (74.1~85.2 MBq/0.5 ml). RESULTS: The carrier-added preparation showed high labeling efficiency (95% at pH 5) and high stability in serum (88%, 3 hr). However, the carrier-free preparation showed low labeling efficiency (59% at pH 5) and low stability (43%, 3 hr). The carrier-added preparation showed high uptake in bone and low uptake in stomach and kidneys. However, the carrier-free preparation showed lower uptake in bone and higher uptake in both stomach and kidneys, which is supposed to be due to released perrhenate. The carrier-added preparation also showed better images with higher skeletal accumulation, lower uptake in other organs and lower soft tissue uptake than the carrier-free preparation. CONCLUSION: The results of these studies clearly demonstrate that addition of carrier perrhenate is required for high labeling efficiency, stability, bone uptake and good image quality of Re-188-HEDP.
