Interaction between fragile histamine triad and protein kinase C alpha in human non-small cell lung cancer tissues
- Author:
Peng-Hui ZHUANG
1
Author Information
1. Department of Thoracic Surgery
- Publication Type:Journal Article
- Keywords:
Co-immunoprecipitation;
Fragile histamine triad (FHIT);
Non-small cell lung cancer (NSCLC);
Protein kinase C alpha (PKCα)
- From:Academic Journal of Xi'an Jiaotong University
2009;21(1):57-61
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the interaction between fragile histamine triad (FHIT) and protein kinase C alpha (PKCα) in human non-small cell lung cancer tissues. Methods: FHIT and PKCα double positive samples were screened by immunohistochemical staining from 13 human non-small cell lung cancer tissues. Co-immunoprecipitation was performed by using anti-FHIT and anti-PKCα. The immune precipitate was analyzed by SDS-PAGE and Western blot. Results: Immune precipitate staining detection showed that 3 samples out of the 13 cases were double positive for FHIT and PKCα. FHIT protein was present in the immune precipitate of anti-PKCα while there was PKCα in the immune precipitate of anti-FHITmAb. Conclusion: FHIT and PKCα exist as a complex in human non-small cell lung cancer tissues, which will provide a new route for studying the pathogenesis and immunotherapy of human non-small cell lung cancer.
