Biological properties of differently-aged human keratinocytes: Population doubling time growth curve and cell cycle analysis
- Author:
Hui-Qun MA
1
Author Information
1. Department of Dermatology
- Publication Type:Journal Article
- Keywords:
Biological property;
Cell cycle;
Grow curve;
Human;
Keratinocyte
- From:Academic Journal of Xi'an Jiaotong University
2009;21(2):129-133
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the biological properties of keratinocytes from differently-aged healthy human beings. Methods: Keratinocytes from fetus, teenager and middle-aged groups were separated and cultured. The population doubling time (PDT) and cell growth curve in different cells were compared, and the cell cycles were analyzed by flow cytometry. Results: Circled digit one In primary culture of keratinocytes, the adherence time in middle-aged group was longer than that in fetus and teenager groups. However, all cell morphology showed no obvious differences. In subculture of keratinocytes, with donator's age increasing, time of cell adherence prolonged, passage number decreased and differences in cell morphology were obvious. Circled digit two The average PDT of keratinocytes was shorter in fetus group than in teenager and middle-aged groups. But difference in cell growth curve between different passages was not observed. Circled digit three Keratinocytes showed G2/M period in fetus group but G0/G1 period in teenager and middle-aged groups mainly. Conclusion: As age increases, the biological properties of keratinocytes change obviously.
