An experimental study of pioglitazone in treating vascular dementia
- Author:
Lu GAO
1
Author Information
1. Emergency Department
- Publication Type:Journal Article
- Keywords:
Immunoenzyme staining;
Immunofluorescence;
Peroxisome proliferator-activated receptor γ;
Pioglitazone;
Vascular dementia
- From:Academic Journal of Xi'an Jiaotong University
2010;22(3):179-182
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the therapeutic effects of different doses of pioglitazone, a kind of peroxisome proliferator-activated receptor γ (PPARγ) agonist, on vascular dementia and explore how pioglitazone affects cerebral ischemia. Methods: Modified Pulsinelli's vessel ligation was used to establish a vascular dementia model in rats. Recognition, learning and memory were evaluated by Morris's water maze test. Immunoenzyme staining was used to determine the number of nerve cells. Immunofluorescence double-staining was used to examine the expression of PPARγ/nerve cells and PPARγ/astrocytes in different groups. Results: Both in pioglitazone groups and sham-operation group, the latency was reduced significantly compared to that in control group (P < 0.01). Sham-operation group had the largest number of neurons in the cortex, followed by low-dose pioglitazone group and high-dose pioglitazone group, and control group came last. Compared with control group, pioglitazone groups had more PPARγ expression in nerve cells, and the fluorescence intensity of PPARγ was stronger. Conclusion: Pioglitazone can induce the expression of PPARγ in neuron endochylema and astrocyte endochylema to protect nerve cells, and then to improve spatial learning and memory function in VD rats.
