Construction of lentivirus vectors carrying alphastatin gene and its secretion expression in human umbilical vein endothelia cells
- Author:
Shi-Wen GUO
1
Author Information
1. Department of Neurosurgery
- Publication Type:Journal Article
- Keywords:
Alphastatin;
Anti-angiogenesis;
Lentivirus;
Vector
- From:Academic Journal of Xi'an Jiaotong University
2010;22(3):168-174
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct lentivirus vectors carrying alphastatin gene, test its secretion expression in human umbilical vein endothelia cells (HUVECs) and observe its effects on growth, migration and tube formation of HUVECs. Methods: We constructed recombinant lentivirus vectors of NT4-alphastatin fusion gene containing neurotrophin-4 signal peptide, pro-region sequences and alphastatin, then transfected the recombinant lentivirus vectors into HUVECs to obtain secretory protein alphastatin and test its anti-angiogenic activities in vitro. Results: Our data showed that recombinant self-inactivating lentivirus vectors of NT4-alphastatin were successfully constructed, and stable NT4-alphastatin transduced HUVECs were capable of sustainably secreting alphastatin which significantly suppressed HUVECs migration and differentiation but not VEGF-induced proliferation. Conclusion: This report represents the first time on the use of lentivirus-based vectors to deliver alphastatin, the endogenous angiogenesis inhibitor, and reveals the potential utility of anti-angiogenic gene therapy with lentivirus vectors for treating cancer.
