Correlation between the expression of P34H and the activity of hyaluronidase in human spermatozoa
10.16098/j.issn.0529-1356.2017.02.013
- Author:
Xiao-Ping MA
1
Author Information
1. Department of Histology and Embryology, Guizhou Medical University
- Publication Type:Journal Article
- Keywords:
Human;
Hyaluronidase;
Immunofluorescence;
Male infertility;
P34H;
Sperm;
Western blotting
- From:
Acta Anatomica Sinica
2017;48(2):193-198
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the correlation between the level of P34H expression and the activity of hyaluronidase in human spermatozoa. Methods: Eighty eight semen samples were collected, 68 cases were in the infertile group, and 20 in the normal control group. Semen routine analysis was referred to the WHO standard method. According to the difference of semen parameters, 68 cases of infertile males were divided into the infertile group with normal semen parameters and the abnormal semen parameters. Western blotting was used to detect the level of P34H expression on spermatozoa. The P34H-positive rate on human spermatozoa was determined by indirect immunofluorescent staining using anti-P34H antibody. The HYD-positive rate and HYD-activity intensity in all samples were examined by improved fixedsubstrate film method. Results: The level of P34H protein expression and the percentage of the P34H-positive rate in infertile groups with normal semen parameters and abnormal semen parameters were significantly lower than that in the control group (P<0.05). The activity of HYD (HYD-positive rate, HYD-activity intensity) in the infertile groups with normal semen parameters and abnormal semen parameters were also significantly lower than that in control group (P < 0.05). The relation between the P34H protein expression and HYD-positive rate, HYD-activity intensity had a significant positive correlation (r = 0. 449, 0. 431; P < 0.01); the relation between the the percentage of P34H-positive rate and HYD-positive rate, HYD-activity intensity had a significant positive correlation (r=0.727, 0.691;P <0.01). Conclusion: The level of P34H protein expression and the percentage of the P34H-positive rate are decreased, while the activity of HYD (HYD-positive rate, HYD-activity intensity) is reduced in male infertility.
