Establishment and identification of radioresistant non-small cell lung cancer model
10.16098/j.issn.0529-1356.2017.02.006
- Author:
Shu-Ya QI
1
Author Information
1. Department of Clinical Medicine, Chengde Medical University
- Publication Type:Journal Article
- Keywords:
Colony formation;
Histochemistry;
Non-small-cell cancer;
Radioresistance
- From:
Acta Anatomica Sinica
2017;48(2):156-159
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a radioresistant model of non-small-cell cancer A549, and to provide the experimental basis for further researchonradioresistance. Methods: The cell number and the dose of radiation therapy were confirmed. The intermittent radiation were used to induce the radioresistant cell model. The morphology of cells were observed with an inverted phase contrast microscope. Colony formation was used to identify the radioresistance of the RA549 cell. Results: RA549 cells were longer and bigger than A549 cells; surviving fraction of RA549 was significantly increased than A549. Conclusion: Intermittent radiation can successfully induce the radioresistant cell model of lung cancer cells.
