Differentiation of human dental pulp stem cells into corneal epithelial-like cells by conditioned medium
10.16098/j.issn.0529-1356.2019.04.021
- Author:
Fang GE
1
Author Information
1. Department of Ophthalmology, Qilu Hospital, Shandong University
- Publication Type:Journal Article
- Keywords:
Conditioned medium;
Dental pulp stem cell;
Flow cytometry;
Humen;
Immunofluorescence
- From:
Acta Anatomica Sinica
2019;50(4):527-532
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the feasibility of inducing human dental pulp stem cells (DPSCs) to differentiate into corneal epithelial-like cells by conditioned medium (CM). Methods: DPSCs were isolated and identified by flow cytometry. The effect of basal medium (BM) and different CM on the proliferation activity of DPSCs was detected by cell counting kit-8(CCK-8) assay. DPSCs were induced by 30%CM, 60%CM, 90%CM. The cells cultured in BM were negative control group. Corneal epithelial cells markers cytokeratin 3 (CK3) and cytokeratin 12 (CK12) were detected by immunofluorescence assay. Results: There was no significant difference in the proliferation activity of DPSCs between BM group and different CM group (P > 0. 05). Cells in the 30%, 60%, 90% CM group did not express CK3 after 3 days induction, cells in the 60%and the 90%CM group began to express CK12; CK3 and CK12 were expressed in the 30%, 60%, 90%CM group after 7 days; At the 11th and 14th day, cells continued to express CK3 and CK12 in the 30%, 60%, and 90% CM groups. No expression of CK3 and CK12 was observed in the BM group. Conclusion: DPSCs are capable of differentiating into corneal epithelial-like cells under the induction of CM.
