Effects of dexmedetomidine in protective effects of propofol on newborn rats hippocampal synapses and the influence of the Bcl-2 expression
10.16098/j.issn.0529-1356.2019.06.003
- Author:
Li YAN
1
Author Information
1. Department of Anesthesiology, Baiyun Hospital Affiliated to Guizhou Medical University
- Publication Type:Journal Article
- Keywords:
Bcl-2;
Dexmedetomidine;
Hippocampal neuron;
Propofol;
Rat;
Real-time PCR
- From:
Acta Anatomica Sinica
2019;50(6):718-721
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the role of dexmedetomidine in protective effects of propofol on newborn rats hippocampal synapses and the influence of the Bcl-2 expression. Methods: Neurons were separated and purificated and identificated by microtubule associated protein-2 (MAP-2) antibody. Rats were divided into normal control group, propofol group(add 12 tng/L propofol), and propofol + dexmedetomidine group (add 12 mg/L propofol and 0.25 mg/L dexmedetomidine). The expression of Bcl-2 test by Real-time PCR and Western blotting; cell synapse was detected by transmission electron microscope. Results: Rat hippocampal neurons were separated successfuly. Hippocampal nerve cells in control group were given priority to with multiple processes of pyramidal cell, the cell body was triangular or elliptic, large cell body bright and pervious to light quality, had a halo around, abundant cytoplasm, nuclear. Compared with the control group, in propofol group the Bcl-2 expression decreased obviously. Compared with the propofol group, the expression of Bcl-2 increased obviously (P<0.05). Conclusion: Dexmedetomidine can influence the expression of Bcl-2 so as to play a protective role of hippocampal neurons.
