The role of N-acetyl-seryl-aspartyl-lysyl-proline in the regulation of inflammatory factor MRP-14 (S100A9) in silicosis rats
- Author:
Hai-Jing DENG
1
,
2
Author Information
- Publication Type:Journal Article
- Keywords: Collagen; Myeloid-related protein14; N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP); Silicosis; Silicosis nodule
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2020;41(1):69-73
- CountryChina
- Language:Chinese
- Abstract: Objective: To explore whether N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP) inhibits the formation of silica nodules and the deposition of collagen by inhibiting the expressions of inflammatory factor myeloid-related protein14 (MRP-14) and collagen in silicosis rats. Methods:The silicosis model of rats was established by one-off bronchial instillation of silicon (SiO2) dust. Sixty healthy adult rats of SPF grade were randomly divided into six groups: control 4-week group, control 8-week group, silicosis model 4-week group, silicosis model 8-week group, Ac-SDKP prevention treatment group, and Ac-SDKP anti-fibrosis treatment group, with ten rats in each group. Immunohistochemical method was used to detect the expression of MRP-14 protein in silicosis model tissues. Western blot method was used to detect the expressions of MRP-14 and collagen protein in silicosis model tissues. Results: Compared with those in the corresponding control group, the expressions of MRP-14 and collagen in silicosis fibrosis areas were significantly increased in the dust-exposed group (P<0.05). Compared with those in the silicosis model 8-week group, the expressions of MRP-14 and collagen were significantly decreased after Ac-SDKP intervention (P<0.05). Conclusion: Ac-SDKP can inhibit silicosis in rats by inhibiting the regulation of MRP-14 expression.
