High expression of death receptor membrane proteins in the cartilage of Kashin-Beck disease
- Author:
Shixun WU
1
Author Information
- Publication Type:Journal Article
- Keywords: Apoptosis; Death receptor protein; DR4/5; Kashin-Beck disease
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2020;41(4):553-558
- CountryChina
- Language:Chinese
- Abstract: Objective To study the changes of apoptosis-related death receptor 4/5 (DR4/5) and FAS expressions in the cartilage from Kashin-Beck disease (KBD). Methods Cartilage specimens of KBD were collected according to the diagnostic criteria of KBD, primary osteoarthrosis (OA) was used as a case control and normal articular cartilage (NC) as a healthy control. The important molecule of DR signaling was detected by immunohistochemical staining. The results were counted under light microscope and the positive rate was calculated. The expression differences of the three groups of cartilage tissues were statistically analyzed. Results The FAS-positive cell rate in the superficial layer of KBD was significantly higher than that in the NC group, and it was higher in OA group than in NC group. The expressions of DR4 and DR5 in KBD group were not significant compared with those in NC group. In the middle layer, both FAS- and DR4-positive cell rates were higher in KBD and the OA cartilage than in the NC control. DR5 expression did not differ between NC group and KBD group. In the deep layer, the FAS positive cell rate in KBD was significantly higher than that in NC, and the DR4 positive cell rate in KBD and OA groups was significantly higher than that in NC group. DR5 did not differ between NC and KBD groups. Conclusion The expression of FAS/DR4 was observed in the deep and middle layers of KBD joints. It suggests that FAS/DR4-mediated cell death may be involved in the characteristic pathological changes of deep cartilage necrosis.
