Bioinformatics analysis of AL360181.1 regulating the progression and prognosis of colorectal cancer
- Author:
Weijun SHI
1
Author Information
- Publication Type:Journal Article
- Keywords: AL360181.1; Bioinformatics analysis; Colorectal cancer; MTOR; Progression and prognosis
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2020;41(5):731-737
- CountryChina
- Language:Chinese
- Abstract: Objective: To analyze the role of gene AL360181.1 in the progression and prognosis of colorectal cancer by bioinformatics method to further predict possible molecular mechanisms. Methods: The colorectal cancer gene expression data and clinical data were downloaded from the TCGA database. The data were analyzed by Perl and R software, and the differentially expressed genes were screened to further analyze the survival, independent prognosis and clinicopathological correlation of differential gene batches. The most relevant differential gene was selected as the target gene; the correlation between the expression of target gene and different clinicopathological features of colorectal cancer and its prognosis were analyzed. The independent prognosis analysis was performed by using R software for different clinicopathological features. The relationship between the target gene and different clinical pathology and patient survival prognosis was analyzed. Finally, GSEA was used to analyze the target gene KEGG pathway and the correlation between target gene and potential target gene was analyzed with R software. We predicted the probable mechanisms of the target gene in regulating the progression and prognosis of colorectal cancer. Results: AL360181.1 was highly expressed in colorectal cancer tissues (P<0.01). AL360181.1 was correlated with the grade and TNM stage of colorectal cancer (P<0.05). Gene AL360181.1, age, tumor grade and TNM staging were associated with the prognosis (P<0.05); gene AL360181.1 and age were used as independent risk factors to evaluate patient prognosis. The expression level of gene AL360181.1, patient age, grade and TNM staging had correlation with the patients' survival status (P<0.05). GSEA enrichment analysis showed that gene AL360181.1 was enriched in mTOR signaling pathway; the correlation analysis of gene AL360181.1 with potential target genes showed that AL360181.1 had correlations with mTOR, AKT, KLKB1, RICTOR, TSC1 and TSC2 (|R|>0.1, P<0.05). Results: Gene AL360181.1 regulates the progression of colorectal cancer through the AKT/mTOR signaling pathway.
