Condition optimization of the flow cytometry MoFlo Astrios EQ on single-cell sorting in 96-well plate
10.3969/j.issn.1674-8115.2018.07.025
- VernacularTitle: 流式细胞仪MoFlo Astrios EQ 96 孔板单细胞分选方法的条件优化
- Author:
Xiao-Cui ZHANG
1
Author Information
1. Core Facility of Basic Medical Sciences, Shanghai Jiao Tong University College of Basic Medical Sciences
- Publication Type:Journal Article
- Keywords:
96-well plate;
Flow cytometry;
Nozzle;
Single-cell culture;
Single-cell sorting;
Straight-down sorting
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2018;38(7):845-850
- CountryChina
- Language:Chinese
-
Abstract:
Objective • To optimize the method of the flow cytometry MoFlo Astrios EQ on single-cell sorting in 96-well plate. Methods • Using different aperture nozzles and sorting ways, the 32D, U937, iBMDM and 293T cells were used for single-cell sorting after the precise adjustment of the instrument and various parameters. The hole numbers with single cell and single-cell clones were detected after sorting. Results • In the single-cell sorting mode, the hole numbers with single cell were 83-91 by 70 μm nozzle and 87-93 by 100 μm nozzle. After 7-10 days of culture, the hole numbers with single-cell clones were 36-58 by 70 μm nozzle. In 100 μm nozzle, the hole numbers with single-cell clones were 53-78 by electrostatic charge sorting and 69-81 by straight-down sorting, respectively. Conclusion • In single-cell sorting, a better cell viability and higher cloning rate are observed in 100 μm nozzle and straight-down sorting.
