Metabolomics study of the effect of arsenic trioxide on hepatocellular carcinoma cell line HepG2
10.3969/j.issn.1674-8115.2018.10.002
- Author:
Jin-Guo HUANG
1
Author Information
1. Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University
- Publication Type:Journal Article
- Keywords:
Arsenic;
Arsenic trioxide;
Hepatocellular carcinoma;
HepG2;
Metabolomics
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2018;38(10):1145-1151
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To reveal the mechanism of the effect of arsenic trioxide (ATO) on hepatocellular carcinoma (HCC) cells through metabolomics study. Methods: HCC cell line HepG2 was treated with ATO for 12 h, 24 h and 36 h respectively. The global metabolite profiles were monitored by metabolomics analysis using GC/MS and LC/MS/MS. Results: A total of 307 certified metabolites were detected. Bioinformatics analysis showed that glycosylation and one-carbon metabolism were the main cellular functions that have been affected. Other affected cellular functions/pathways included mitochondrial function fatty acid β-oxidation, TCA cycle, pentose phosphate pathway (PPP) and glutathione metabolism. Conclusion: ATO treatment affects the main metabolomic pathway of HepG2, and inhibits cell growth and migration, then exacerbates oxidative stress to induce cancer cell apoptosis.
