Generation and phenotypic analysis of Fbxo22 knockout mice
10.3969/j.issn.1674-8115.2019.04.004
- Author:
Hui-Lin ZHANG
1
Author Information
1. Center of Molecular Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine
- Publication Type:Journal Article
- Keywords:
CRISPR-Cas9 technology;
Early postnatal lethality;
FBXO22
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2019;39(4):353-357
- CountryChina
- Language:Chinese
-
Abstract:
Objective • To establish the Fbxo22 knockout mouse model and study the biological function of FBXO22. Methods • The Fbxo22 knockout mice were generated by CRISPR-Cas9 technology. The number, appearance, weight of different embryos and mice were measured. Meanwhile, the food intake and survival of Fbxo22-/- mice were analyzed. Results • Although the Fbxo22-/- embryos were present at approximately Mendelian ratios on embryonic day 17.5/18.5, most of them died within 48 hours of birth. Furthermore, those surviving Fbxo22-/- mice showed reduced body size and food intake and decreased life span. Conclusion • FBXO22 is an important, albeit not essential, protein for early postnatal survival and normal development.
