Effects of lutein on transforming growth factor-β2 induced epithelial-mesenchymal transition in ARPE-19 cells
10.3969/j.issn.1674-8115.2019.06.003
- Author:
Ya-Nan LÜ
1
Author Information
1. Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai Key Laboratory of Ocular Fundus Disease, Department of Ophthalmology, Shanghai General Hospital,Shanghai Jiao Tong University School of Medicine
- Publication Type:Journal Article
- Keywords:
Epithelial-mesenchymal transformation (EMT);
Lutein;
Retinal pigment epithelium cell (RPE);
Subretinal fibrosis;
Transforming growth factor-β2 (TGF-β2)
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2019;39(6):571-577
- CountryChina
- Language:Chinese
-
Abstract:
Objective • To establish the transforming growth factor-β2 (TGF-β2) induced epithelial-mesenchymal transition (EMT) model of retinal pigment epithelium cells, and investigate the effect and mechanism of lutein on EMT. Methods • ARPE-19 cells were cultured and divided into 4 groups including control group, TGF-β2 group, TGF-β2+lutein group and lutein group. The mRNA levels of α-smooth muscle actin (α-SMA), fibronectin (FN) and E-cadherin were analyzed by real-time PCR. The protein expression of α-SMA, FN and occludin were assayed by Western blotting. Immunofluorescence was used to detect the change of α-SMA. Meanwhile, Western blotting was performed to detect the expression levels of pSmad3 in the TGF/Smad signaling pathway. Results • TGF-β2 induced EMT was inhibited by lutein. Lutein decreased the mRNA and protein levels of the mesenchymal markers α-SMA and FN, and increased the expression of the epithelial markers E-cadherin and occludin (all P<0.05). Immunofluorescence showed that lutein can inhibit the conversion of epithelial cells into myofibroblasts. Lutein significantly downregulated the high expression of pSmad3 in TGF-β2 treated ARPE-19 cells (P=0.001). Conclusion • Lutein inhibits TGF-β2 induced EMT by downregulating the expression of pSmad3 in TGF-β/Smad signaling pathway, indicating it may attenuate subretinal fibrosis.
