Fabrication of an antibacterial hydrogel laden with adhesive liposomes for bone repairment
10.3969/j.issn.1674-8115.2019.09.003
- Author:
Yi XIANG
1
Author Information
1. Institute of Orthopaedics, Soochow University
- Publication Type:Journal Article
- Keywords:
Adhesive liposome;
Antibacteria;
Bone repair;
Hydrogels
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2019;39(9):947-954
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To construct an antibacterial hydrogel laden with adhesive liposomes (A-LIP-PEG) for bone repair. Methods: Adhesive liposomes (A-LIP) were fabricated based on the Schiff-based reaction between dopamine and the liposome. To prepare the liposome composite hydrogel, the A-LIPs were mixed with thiolated polyethylene glycol (4SH-PEG) which was then crosslinked with Ag+. Bone morphogenetic protein 2 (BMP-2) was loaded in the A-LIPs for its uniform dispersion and sustained release in the hydrogel network. The size distribution and zeta-potential of the A-LIPs were characterized with a particle size analyzer. The morphology of the A-LIPs was observed under transmission electron microscope. Flushing test was employed to examine the viscidity of the A-LIPs, and CCK-8 assay was conducted to demonstrate the biocompatibility of the A-LIP-PEG. Osteogenic activity of the A-LIP-PEG was evaluated by alkaline phosphatase assay and alizarin red S staining in bone marrow mesenchymal stem cells (BMSCs). Results: Strong adhesion toward soft tissue of the A-LIPs was indicated by in vitro study. A-LIP-PEG showed significant inhibition on Staphylococcus aureus. A-LIP-PEG showed superior promotion of osteogenic differentiation to hydrogel laden with liposomes (LIP-PEG), without disrupting cell proliferation. Conclusion: The A-LIP-PEG developed in this study shows notable potential to expand the application of hydrogels to drug delivery, antibacteria and bone repair.
