Biological characteristics and gene expression profile analysis of two kinds of mouse fetal liver stromal cells
10.3969/j.issn.1674-8115.2019.11.002
- Author:
Yun-Han WANG
1
Author Information
1. Department of Histoembryology, Genetics & Development, Shanghai Jiao Tong University College of Basic Medical Sciences
- Publication Type:Journal Article
- Keywords:
Fetal liver;
Flow cytometry;
Hematopoietic stem cell (HSC);
Ingenuity pathway analysis (IPA);
Microarray;
Stromal cell
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2019;39(11):1226-1232
- CountryChina
- Language:Chinese
-
Abstract:
Objective • To explore the differences in morphology, molecular characteristics and biological functions of different types of mouse fetal liver stromal cells. Methods • E13.5 mouse fetal liver stromal cells were obtained by adherent culture, and different cell types were distinguished by morphology and expression of surface markers, such as CD44, CD29, CD106, CD45 and Sca-1. Then microarray assay was conducted by Mouse Genome 430 2.0 Array and analyzed at P<0.05, fold change>3 or <-2 to identify differential expression genes. Canonical pathway and biological function analyses were performed by using ingenuity pathway analysis (IPA software). Results • Spindle-like (CD45+CD106-CD29+CD44+Sca-1-) and fibroblast-like (CD45-CD106+CD29+CD44+Sca-1-) fetal liver stromal cells were isolated in this study according to the cell morphology. The 1 485 highly-expressed genes in spindle-like cells mainly involved in immune and inflammation-related signaling pathways; while the 3 374 highly-expressed genes in fibroblast-like cells mainly involved in extracellular matrix formation and cellular adhesion. Conclusion • Mouse fetal liver stromal cells have strong heterogeneity in biological characteristics and functions, especially in hematopoietic promoting capability.
