Effect of Lycium barbarum polysaccharide and aerobic exercise on rats with non-alcoholic fatty liver disease and its mechanism
10.3969/j.issn.1674-8115.2020.01.005
- Author:
Yi-Qiong GUO
1
Author Information
1. School of Public Health and Management, Ningxia Medical University, Ningxia Hui Autonomous Region
- Publication Type:Journal Article
- Keywords:
Aerobic exercise;
High fat diet;
Lycium barbarum polysaccharides;
Non-alcoholic fatty liver disease
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2020;40(1):30-36
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of different doses of Lycium barbarum polysaccharides (LBP) and aerobic exercise on rats with non-alcoholic fatty liver disease (NAFLD) and its mechanisms. Methods:SD rats of 8 weeks old were randomly divided into normal control group and high fat diet (HFD) group. The normal control group was given normal diet, and the HFD group was given HFD for 10 weeks to construct a NAFLD rat model. After successful modeling, the rats with NAFLD were assigned into four groups with eight in each, namely: HFD group, LBP-a group (50 mg/kg), LBP-b group (100 mg/kg) and aerobic exercise group (20 m/min, 60 min/d). After the intervention for 6 weeks, fasting for 12 hour and fasting blood glucose level was measured. Blood samples and liver tissues of rats were collected after intraperitoneal anesthesia with 10% chloral hydrate. The levels of glutamate pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), total cholesterol (TC), triacylglycerol (TAG), low density lipoprotein (LDL), high density lipoprotein (HDL), malondialdehyde (MDA), superoxide dismutase (SOD) and irisin in rats were measured according to corresponding protocols of kits. The expressions of peroxisome proliferators-activated receptor-γ coactivator-1 α (Pgc1-α) and fibronectin type III domain-containing protein 5 (Fndc5) in liver were detected by using reverse transcription polymerase chain reaction. Results:Compared with the HFD group, the levels of GPT, GOT, TC, TAG, MDA and homeostasis model assessment of insulin resistance (HOMA-IR) were significantly decreased in LBP-a group, LBP-b group and aerobic exercise group (P<0.05). The levels of HDL and SOD were significantly increased in three intervention groups (P<0.05). The levels of LDL were markedly decreased and the levels of irisin were significantly increased in LBP-b group and aerobic exercise group compared with the HFD group (P<0.05). The expressions of Pgc1-α mRNA in LBP-b group and aerobic exercise group were up-regulated as compared with the HFD group (P<0.05). The expressions of Fndc5 mRNA in three intervention groups were up-regulated as compared with the HFD group (P<0.05). Conclusion:LBP and aerobic exercise can significantly improve lipid metabolism disorder and decrease liver oxidative stress in rats with NAFLD, which may be related to the up-regulated expressions of Pgc1-α mRNA and Fndc5 mRNA.
