Expression and clinical significance of α1-antitrypsin in thyroid-associated ophthalmopathy
10.3969/j.issn.1674-8115.2020.04.003
- VernacularTitle: 甲状腺相关眼病泪液α1-抗胰蛋白酶的表达量及临床意义
- Author:
Yu WU
1
Author Information
1. Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology
- Publication Type:Journal Article
- Keywords:
Enzyme-linked immunosorbent assay (ELISA);
Tears;
Thyroid-associated ophthalmopathy (TAO);
Α1-antitrypsin (α1-AT)
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2020;40(4):430-436
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the expression and clinical significance of α1-antitrypsin (α1-AT) in tears of thyroid-associated ophthal-mopathy (TAO) patients. Methods ¡¤ Patients diagnosed with TAO at Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine from January to December 2019 were included and divided into active TAO group (n=33) and inactive TAO group (n=22). Thirty eyes of 30 healthy volunteers were included (n=30). Unstimulated tear samples were collected from all subjects and the concentration of α1-AT was analyzed by enzyme-linked immunosorbent assay (ELISA). Difference of α1-AT concentration among 3 groups was compared by using Kruskal-Wallis H test. Mann-Whitney U test was performed for further comparison between each 2 groups. Correlationship between α1-AT con-centration and clinical activity score (CAS) was analyzed by Spearman relation. Statistical significance was accepted at a value of P<0.05. Re-sults ¡¤ α1-AT was significantly higher in active TAO group than that in inactive TAO group and control group (both P<0.05). There was no significant difference in α1-AT between inactive TAO group and control group. Tear α1-AT level was significantly correlated with CAS (r=0.846, P=0.000). ROC curve showed that the optimal diagnostic cut off value of α1-AT was 939.48 ng/mL, and the sensitivity and specificity were 81.8% and 100% (AUC=0.959, P<0.05). Conclusion ¡¤ α1-AT is significantly elevated in the tears of active TAO patients. Tear α1-AT level has important value in the diagnosis of clinical activity of TAO.
