Inhibitory effect of toosendanin on gastric cancer cells BGC-823 by downregulating circDLST
10.3969/j.issn.1674-8115.2020.09.006
- VernacularTitle: 川楝素通过下调环状RNA circDLST对胃癌细胞BGC-823的抑制作用
- Author:
Jing ZHANG
1
Author Information
1. Department of Gastroenterology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University
- Publication Type:Journal Article
- Keywords:
CircDLST;
Circular RNA;
Gastric cancer;
Toosendanin;
Tumor growth;
Tumor invasion
- From:
Journal of Shanghai Jiaotong University(Medical Science)
2020;40(9):1202-1206
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of toosendanin (TSN) on the growth and invasion of gastric cancer cells BGC-823 by regulating circDLST expression. Methods: After gastric cancer cells BGC-823 were exposed to different con-centrations of TSN (0, 0.5, 1.0, and 2.0 μmol/L) for 24 h, quantitative PCR was used to detect the expression of circDLST. BGC-823 cells were transfected with the circDLST overexpression lentiviral vector or its control vector (CON), and then treated with 0.5 μmol/L TSN or PBS. So the cells were divided into circDLST+TSN group, CON+TSN group and CON+PBS group. The viability and invasive potential of BGC-823 cells were observed by MTT proliferation test and Transwell invasion assay. The subcutaneous transplanted tumor models were established by using circDLST-transfected cell line BGC-823 or the control cell line in nude mice, and then 200 μg/kg TSN or the same volume of PBS was injected intraperitoneally every day. So the mice were divided into circDLST+TSN group, CON+TSN group and CON+PBS group. Results: Compared with the control group (0 μmol/L), all 3 concentrations of TSN decreased the expression levels of circDLST in a concentration dependent manner (P<0.01). TSN could significantly reduce the cell viability, cell invasion and subcutaneous xenograft tumor growth (P=0.000), while circDLST overexpression reversed the inhibitory effect of TSN (P<0.01). Conclusion: TSN may inhibit the growth and invasion of gastric cancer cells BGC-823 by downregulating circDLST expression.
