Simultaneous determination of four active ingredients in wuji pellet by HPLC
10.1016/S1674-6384(13)60044-1
- Author:
Qun WANG
1
Author Information
1. Guiyang College of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Berberine;
Evodiamine;
Paeoniflorin;
Rutaecarpin;
Wuji Pellet
- From:
Chinese Herbal Medicines
2014;5(4):297-300
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To apply the high performance liquid chromatography (HPLC) method to the determination of four ingredients including paeoniflorin, berberine, evodiamine, and rutaecarpin in Wuji Pellet. Methods: Using Thermo-ODS2-Hypersil column (250 mm × 4.6 mm, 5 μm) and taking acetonitrile-0.05 mol/L monopotassium phosphate as mobile phase for elution, the conditions were as follows, flow rate of 1.0 mL/min, UV detector wavelength of 225 nm, and column temperature of 25 °C. Results: The standard curves of paeoniflorin, berberine, evodiamine, and rutaecarpine showed a good linear relationship in 0.0124-0.124, 0.0349-0.349 mg/mL, and 0.6-6, 0.5-5 μg/mL. The average recovery rates were 99.45%, 97.69%, 98.18%, and 98.46%, respectively. Conclusion: The method is of high sensitivity and nice reproducibility, and it could be used for the simultaneous determination of the four ingredients in Wuji Pellet. © 2013 Tianjin Press of Chinese Herbal Medicines.
