Screening of phytoestrogenic effective extracts and dose of cistanche deserticola
10.1016/S1674-6384(13)60043-X
- Author:
Wen-lan LI
1
Author Information
1. Research Center on Life Sciences and Environmental Sciences
- Publication Type:Journal Article
- Keywords:
Cistanche deserticola;
Effective extracts and dose;
MTT;
Phytoestrogens;
Uterus growth test
- From:
Chinese Herbal Medicines
2014;5(4):292-296
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To screen the phytoestrogenic effective extracts and dose of Cistanche deserticola including estrogenic and anti-estrogenic activities. Methods: The effect of phytoestrogen was determined through uterus growth test in low and high estrogen female model mice. Then MTT assay of the estrogen-dependent breast cancer cells MCF-7 was conducted with the medicated serum of mice. Results: After ig administration with 95% ethanol extract of C. deserticola [EECD, 30 g/(kg·d)], the uterus coefficient of low estrogen model mice increased. The medicated serum of 30 g/(kg·d) EECD significantly promoted the proliferation of MCF-7 cells. 40 g/(kg·d) EECD + diethylstilbestrol significantly inhibited the growth of the uterus in high estrogen model mice and the proliferation of MCF-7 cells as well. Conclusion: With the dose of 30 g/(kg·d), EECD could exert quasi estrogen effect, and with the dose of 40 g/(kg·d), EECD could exert the estrogen antagonistic action. The method established is accurate and reliable, which could be used for the follow-up studies on the phytoestrogen material basis of C. deserticola. © 2013 Tianjin Press of Chinese Herbal Medicines.
