Proteomic Profiling of Human Small Cell Lung Cancer Cell Line NCI-H211.
- Author:
Hee Young CHO
1
;
Mi Kyung KIM
;
Young Do YOO
;
Myung Ju AHN
;
Joung Soon JANG
Author Information
1. Department of Internal Medicine, GyeongSang National University College of Medicine and GyeongSang Institute of Health Sciences, Jinju, Korea. jsjang@nongae.gsnu.ac.kr
- Publication Type:Original Article
- Keywords:
Proteome;
Small cell lung cancer;
Reference map
- MeSH:
Cause of Death;
Cell Line*;
Cytoskeletal Proteins;
Dermatoglyphics;
Electrophoresis, Gel, Two-Dimensional;
Electrophoresis, Polyacrylamide Gel;
Eukaryotic Cells;
Humans*;
Isoelectric Focusing;
Mass Spectrometry;
Peptides;
Proteolysis;
Proteome;
Proton-Motive Force;
Silver Staining;
Small Cell Lung Carcinoma*
- From:Cancer Research and Treatment
2003;35(6):489-496
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Small cell lung cancer is one of the major causes of death from cancer worldwide. To explore the expressions of global protein in small cell lung cancer cells, a proteomic approach, to identify the proteins, was used and the establishment of a protein reference map attempted. MATERIALS AND METHODS: Two-dimensional gel electrophoresis (2-DE), with subsequent analysis by mass spectrometry (MS), was applied to the study of protein identification from a small cell lung cancer cell line, NCI- H211. The cells were lysed, and the extracts subjected to isoelectric focusing, with immobilized pH gradients, followed by second dimension SDS-PAGE. The polypeptides were identified by peptide mass fingerprinting, with MALDI-TOF MS, after in-gel protein digestion. RESULTS: From silver staining of the gel, around two thousands protein spots were separated by the 2-DE. Of these protein spots visualized in the gel, one hundred and ten were identified by peptide mass fingerprinting. Different proteins, such as enzymes, cytoskeletal proteins and proteins common to eukaryotic cells, were identified. CONCLUSION: The protein expressions of the small cell lung cancer cells were analyzed to establish a protein reference map. The reference map presented here may serve as a working tool for the further study of small cell lung cancer.
