Effect of VEGF165b on biological characteristics of human hepatocellular carcinoma HepG2 cells
10.13481/j.1671-587x.20180119
- Author:
Yanying ZHAO
1
Author Information
1. Department of Gastroenterology, Fourth Hospital, Jilin University
- Publication Type:Journal Article
- Keywords:
Cell migration;
Cell proliferation;
HepG2 cells;
Liver neoplasms;
Vascular endothelial growth factor 165;
Vascular endothelial growth factor 165b
- From:
Journal of Jilin University(Medicine Edition)
2018;44(1):101-105
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of vascular endothelial growth factor 165b (VEGF165b) on the biological characteristics of human hepatocellular carcinoma HepG2 cells, and to explore its mechanism. Methods: The HepG2 cells were divided into blank group (treated with transfection reagent), control group (transfected with pcDNA3. 0 expression vector) and pcDNA-VEGF165b group (transfected with pcNDA-VEGF165b). MTT assay was used to detect the survival rate of HepG2 cells; RT-PCR and Western blotting method were used to detect the expression levels of VEGF165b and VEGF165 mRNA and protein in the HepG2 cells; Transwell chamber assay was used to measure the migration ability of HepG2 cells. Results: Compared with blank group, there was no significant changes in the expression levels of VEGF165b and VEGF165 mRNA and protein in the HepG2 cells in control group (P>0. 05). Compared with blank group, the expression levels of VEGF165b mRNA and protein in the HepG2 cells in PcDNA-VEGF165b group were significantly increased (P< 0. 05), and the expression levels of VEGF165 mRNA and protein were significantly decreased (P<0. 05). There was no significant difference in the survival rates between blank group and control group (P>0. 05). The survival rate of HepG2 cells in PcDNA-VEGF165b group was lower than those in blank group and control group, but the differences were not statistically significant (P>0. 05). The cell migration experiment results showed that there was no significant difference in the migration rate between blank group and control group (P<0. 05). Compared with blank group and control group, the migration rate of HepG2 cells in PcDNA-VEGF165b group was significantly reduced (P<0.05). Conclusion: VEGF165b can inhibit the expressions of VEGF165 mRNA and protein, and VEGF165b has no effect on the proliferation of hepatocellular carcinoma cells; but it can reduce the migration of hepatocellular carcinoma cells.
