Inhibitory effect of scutellarin on proliferation of human glioma U87 cells and its mechanism
10.13481/j.1671-587X.20180302
- Author:
Peng LYU
1
Author Information
1. Department of Pathophysiology, Jilin Medical College
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Bax;
Bcl-2;
Cell proliferation;
Glioma;
Scutellarin
- From:
Journal of Jilin University(Medicine Edition)
2018;44(3):466-470
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the inhibitory effect of scutellarin (SCU) on the proliferation of human glioma U87 cells, and to clarify its mechanism. Methods: The U87 cells were cultured and divided into blank control group and 20, 40, 80 mg · L-1 SCU groups, the surival rates of U87 cells in various groups were examined by MTT assay; the morphology of U87 cells in various groups were observed by inverted microscope; the apoptotic rates of U87 cells were examined by Annexin V/PI staining; the expression levels of bax and bel-2 proteins in the U87 cells in various groups were detected by Western blotting method. Results: The MTT results showed that compared with blank control group, the survival rates of U87 cells in 20, 40, and 80 mg · L-1 SCU groups were decreased (P
