Structures of ginseng glycopeptides and anti-apoptotic effect on PC12 cells treated with Aβ25-35
10.13481/j.1671-587x.20190213
- Author:
Jingting HU
1
Author Information
1. School of Pharmacy, Changchun University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Amyloid beta;
Apoptosis;
Cell cycle;
Glycopeptide;
Panax ginseng
- From:
Journal of Jilin University(Medicine Edition)
2019;45(2):286-293
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the structures of ginseng glycopeptides, and to explore the protective effect on the apoptosis of PCI 2 cells treated with amyloid beta25-35 (Aβ25-35), and to lay a theoretical foundation for the development of ginseng anti-Alzheimer' s disease (AD) drugs. Methods: The structures of ginseng glycopeptides were analyzed by reversed-phase high performance liquid chromatography coupled with Q Exactive Orbitrap mass spectrometry. The PCI2 cells were divided into 6 groups, and treated with the medium including different concentrations 0, 6. 25, 12. 50, 25. 00, 50. 00, and 100. 00 mol • L_ 1 ) of Aβ25-35 , and the survival rates of PC12 cells were measured by cell counting kit-8 (CCK-8) method. The PC12 cells were divided into blank control group, model group, and ginseng glycopeptides administration group. The medium including 50. 00 jumol • L_ 1 Aβ25-35, was added in model group, and different concentrations 0. 03, 0.10, 0. 30, and of 1. 00 g • L_ 1 ) ginseng glycopeptides+ 50 jumol • L_ 1 Aβ25-35 were added in administration groups. The survival rates of PC12 cells were measured by CCK-8 method. The apoptotic rates of PCI 2 cells after treated with glycopeptides and Aβ25-35, were detected by Annexin V-FITC/PI method. The PCI 2 cells were divided into blank control group, model group (50.00 jumol • L_ 1 Aβ25-35) and different concentrations (0 . 1 0 and 0.30 g • L_ 1 ) of ginseng glycopeptides administration groups; the percentages of PCI 2 cells in different cell cycles were tested by flow cytometry. Results: More than 20 glycopeptide structures were obtained, such as the peptide chain was NLSHYHSGSS, the glycosyl group was Nl-HexNAc, and the peptide chain was SGSSSSSSSEDDGMGR, the glycosyl group was S6-HexNAc. When the concentration of Aβ25-35 was 50 jumol • L_ 1 , the survival rate of PC12 was (55. 45 + 2. 3 4) % and the survival rate was significantly lower than that in blank control group (P < 0 . 0 1) . Compared with blank control group, the survival rate of the PC12 cells in model group was significantly decreased (P < 0 01); compared with model group, the survival rates of the PCI2 cells in administration groups were significantly increased (P< 0. 05). Compared with blank control group, the apoptotic rate of the PC12 cells in model group were increased (P < 0. 01); compared with model group, the apoptotic rates of the PC12 cells in administration groups were decreased (P < 0 . 05). Compared with blank control group, the percentage of PC12 cells in S phase in model group was increased (P
