Effect of silencing mitochondrial ribosomal protein l35 gene on growth of human esophageal cancer te-1 cells
10.13481/j1671587x20190106
- Author:
Aifu WANG
1
Author Information
1. Department of Radiotherapy, Second Hospital, Jilin University
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Esophageal cancer te-1 cells;
Mitochondrial ribosomal protein 1-35
- From:
Journal of Jilin University(Medicine Edition)
2019;45(1):28-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of lentivirus-mediated silencing of mitochondrial ribosomal protein L35 (MRPL35) gene on the growth of human esophageal cancer TE-1 cells, and to clarify its mechanism Methods: Three kinds of human esophageal cancer cells, TE-1, ECA109 and KYSE150, were selected. The relative expression levels of MRPL35 mRNA in three kinds of cells by real-time quantitative PCR. The esophageal cancer TE-1 cells were divided into sh MRP 1.35 group and shCtrl group, and the cells were infcctcd with si-RNA lentivirus and si-RNA lentivirus; the esophageal cancer cell line stably silenting the MRPL35 gene was established. Real-time quantitative PCR and Western blotting methods were used to detect the efficiency of MRPL35 gene silencing. The cell growth curves in various groups were detected by CCK-8 method, and the apoptotic rates were detected by flow cytometry after Annexin \ -PE/7AAD double staining. Results: Three kinds of esophageal cancer cells expressed MRPL35 gene, and the expression levels were not statistically significant between them (P>0.05). The results of real-time quantitative PCR and Western blotting methods showed that the mRNA and protein levels of MRPL35 in the TE-1 cells in shMRPI.35 group were significantly lower than those in shCtrl group ( P<∗0. 05). Compared with shCtrl group, the cell growth speed in shMRPI.35 group was decreased ( P
