Effect of periodontitis patient s own tissue nucleic acid on expression of nlrp3 mRNA in murine macrophages and its significance
10.13481/j.1671-587x.20190105
- Author:
Zhongzhcng YUAN
1
Author Information
1. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling
- Publication Type:Journal Article
- Keywords:
Inflammation-related factors;
Macrophages;
Nod-like receptor protein 3;
Periodontitis: Own tissue nucleic acids
- From:
Journal of Jilin University(Medicine Edition)
2019;45(1):23-27
- CountryChina
- Language:Chinese
-
Abstract:
Objcctivc: To detect the mRNA expression leveb of inflammatory-related factors NLRP3, caspase-1. IL-lp. and 1L-18 in the murine macrophages infected by periodontitis patient's own tissue nucleic acid, and to discuss the effects of penodontitis patient' s own tissue nucleic acid on the inflammation-related factors in the macro. hacs. Methods: The mflammator. . eriodontal tissue sam. les were collected durin . eriodontal fla. surcr. of the chronic periodontitis patients, and the healthy periodontal tissue samples were collected from the patients without any periodontal diseases undergoing crown lengthening surgery. Then the total RNA from gingival tissue was extracted and reversely transcribed into cDNA. The cultured mouse macrophages RAW264. 7 were divided into control group and experiment group, then the healthy periodontal tissue cDNA and inflammatory periodontal tissue cDNA (the cDNA at a concentration of 1 mg • L ) were added into the RAW264. 7 cells, respectively. Real-time PCR was used to detect the mRNA expression levels of NLRP3 . Caspase-1. 1L-1(3. and 1L-18 in the macrophages in various groups at 4. 6 and 8 h after incubation. Rcsilts: The microscope observation showed that the mouse macrophages RAW264. 7 grew well with round and polygon shapes, clear cytoplasm, and full cell body. Compared with control group, the expression levels of NLRP3. Caspase-1. 1L-10. and 1L-18 mRNA in the RAW264. 7 cells in experiment group at 4. 6. and 8 h were increased significantly ( P<0. 05 or P<0. 01). and the expresaon levels of NLRP3. Caspase-1. 1L-10. and 1L-18 mRNA in RAW264.7 cells at 6 h in experiment group were the highest. Ccoclusico. The periodontitis patient's own tissue nucleic acids can promote the mRNA expressions of inflammation- related factors in the RAW264. 7 cells, suggesting that the periodontitis patient' s own tissue nucleic acid has an immunomodulatory effect on the activation of RAW264. 7 cells.
