Transcriptome sequencing and analysis of bovine alveolar macrophages infected with bcg
10.13481/j.1671-587x.20190103
- Author:
Jinrui XU
1
Author Information
1. Ningxia University, Key laboratory of Protection, Utilization of Special Biological Resources in Western China, Ministry of Education
- Publication Type:Journal Article
- Keywords:
Bovine alveolar macrophage;
Long non-coding;
Racillus calmette-guerin;
Transcriptomc sequencing
- From:
Journal of Jilin University(Medicine Edition)
2019;45(1):12-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To reveal the differentially expressed genes and long non-coding RNAs (IncRNAs) by sequencing the transcriptome of bovine alveolar macrophages (RAM) infected with Bacillus Calmette-Guerin (RCG) and analyzing their bioinformations, and to provide theoretical foundation for the research on immune regulation mechanism of anti-infection of Mycobacterium tuberculosis of macrophages. Methods: The RAM were collected by pulmonary lavage and centrifligation and cultured and divided into infected group and uninfected group. After infection for 12 h in infected group, the expression profiles of mRNA and IncRNA in infected group and uninfected group were detected by RNA-Seq, and bioinformatics analysis was carried out. Results: Compared with uninfected group, there were 119 differentially expressed IncRNAs and 1111 differentially expressed mRNA in infected group (P
