Inhibitory effect of lumbrokinase on proliferation and induction on apoptosis of gastric cancer sgc7901 cells
10.13481/j.I671-587x.20190102
- Author:
Dan LIU
1
Author Information
1. Department of Pharmacy
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Cell cycle;
Cell scratch;
Lumbrokinase;
Sgc7901 cells
- From:
Journal of Jilin University(Medicine Edition)
2019;45(1):7-11
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the regulatory effect of lumbrukinase CI.RK) on the gastric cancer SGC7901 Cells, and to clarify its mechanism Methods: The SGC7901 cells in the logarithmic growth phase were selected and divided into control group and 2, 4 , 8 U • ml. I.RK groups. MTT assay was used to detect the inhibitory rates of proliferation of SGC7901 cells in various groups in vitro at different time (24, 48 and 72 h). Cell scratch assay was used to detect the migration abilities of the SGC7901 cells in vitro in various groups. Flow cytometry was used to determine the apoptotic rates of SGC7901 cells and the pencentages of cells at different cell cycles in various groups. The expression levels of Bcl-2, Rax, and caspase-3 in the SGC790I cells in various groups were detected by Western blotting method. Results: The results of MTT assay showed that compared with control group, the inhibitory rates of SGC7901 cells in different doses of I.RK groups after treated for 24, 48 and 72 h were increased ( P<0.01). The cell scratch assay results showed that compared with control group, the migration distances of SGC790I cells in 4 and 8 U • mL"1 I.RK groups were increased significantly ( P
