Differential expression of eriCF gene in fluoride-resistant strains of Streptococcus mutans and its signficance
10.13481/j.1671-587x.20190314
- Author:
Tian QU
1
Author Information
1. Department of Operative and Endodontics, Stomatology Hospital, Jilin University
- Publication Type:Journal Article
- Keywords:
eriCF1;
eriCF2;
Fluoride-resistant strains of streptococcus mutans;
Fluorine resistance;
Streptococcus mutatis
- From:
Journal of Jilin University(Medicine Edition)
2019;45(3):551-557
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effects of eriCF1 and eriCF2 genes under the condition of different fluoride concentrations on the fluoride resistance of Streptococcus mutans (S. mutans) fluoride-resistant strain UA159-FR, and to elucidate the relationship between the differential expressions of eriCF genes and the fluoride resistance of S. mutans fluoride-resistant strain. Methods: The recombinant E. coli competent cells BL21 were divided into eriCF1 group (eriCF1+ Peasy-Blunt E2), eriCF2 group (eriCF2+ Peasy-Blunt E2) and KZ group (Peasy-Blunt E2). After gene cloning and IPTG induction, the expression levels of EriCF1 and EriCF2 proteins in three groups were detected, and the concentrations of bacterial solution and the growth rates of bacterium of strains in three groups were determined under different concentrations of fluoride conditions (1. 5, 2.0, 2.5, and 3. 0 g middot; L-1 sodium fluoride); the growth curves were drawn. Results: At logarithmic phase (8 h) and stable phase (16 h), the final concentrations of bacterial solution and the growth rates of the strains in three groups under different concentrations of fluorine environments (1. 5, 2. 0, 2. 5 and 3. 0 g middot; L-1 sodium fluoride) were compared in the same group, and the final concentrations and the growth rates of bacterial solution of the strains in three groups were decreased with the increasing of sodium fluoride concentration; the final concentrations and the growth rates of bacterial solution results were eriCF2 group > eriCF1 group > KZ group (P<0. 05 or P<0. 01).compared between three groups under the same concentration of sodium fluoride, the concentration of bacterial solution in eriCF2 group was increased significantly compared with eriCF1 group (P<0. 01). Conclusion: Both eriCF1 and eriCF2 genes can enhance the fluoride resistance of E. coli, and the fluoride resistance of E. coli expressing eriCF2 gene is stronger than that of E. coli expressing eriCF1 gene.
