Promotion effect of ursolic acid on differentiation of mouse cementoblast cell line occm-30 cells
10.13481/j.1671-587x.20190502
- Author:
Menghong LI
1
Author Information
1. Department of Orthodontics, Stomatology Hospital, Jilin University
- Publication Type:Journal Article
- Keywords:
Cell differentiation;
Cell proliferation;
Cementoblast;
Ursolic acid
- From:
Journal of Jilin University(Medicine Edition)
2019;45(5):986-991
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects of ursolic acid on the differentiation of cementoblast cell line OCCM-30 cells, and to provide the theoretical basis for the repair of root restoration. Methods: The OCCM-30 cells in logarithmic growth phase were divided into control group and different concentrations of ursolic acid groups. The OCCM-30 cells in ursolic acid groups were treated with different concentrations (0.625, 1.250 and 2. 500 μmol · L-1) of ursolic acid and the cells in control group did not receive any treatment. MTT method was performed to detect the inhibitory rates of proliferation of the OCCM-30 cells in various groups at different time points (24, 48, and 72 h); ALP (alkaline phosphatase) assay was performed to detect the cell differentiation and mineralization; real-time quantitative PCR was used to determine the expression levels of osteopontin (OPN) mRNA during 24 h; the expression levels of OPN protein at 3 and 5 d after administration were detected by Western blotting method. Results: There were no significant differences in the inhibitory rates of proliferation of OCCM-30 cells between control group and different concentrations of ursolic acid groups (P>0. 05). Compared with control group, the ALP activities in the OCCM-30 cells in 2. 500 μmol · L-1 ursolic acid group at 3, 5, and 7 d after administration were significantly increased (P<0. 05). Compared with control group, the expression levels of OPN mRNA and protein in the OCCM-30 cells in different concentrations of ursolic acid groups were significantly increased (P<0. 01). Conclusion: Appropriate concentration of ursolic acid has no effect on the inhibitory rate of proliferation of OCCM-30 cells, but it can promote the differentiation of OCCM-30 cells and up-regulate the expression levels of OPN mRNA and protein.
