Induction of LPS on epithelial mesenchymal transition in breast cancer MDA-MB-231 cells and its effect on β-catenin expression
10.13481/j.1671-587x.20200217
- Author:
Zhouhua CHEN
1
Author Information
1. Department of Oncology, Second People' s Hospital of Xiangtan City
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Epithelial mesenchymal transition;
Lipopolysaccharide;
Wnt/fi-catenin signaling pathway
- From:
Journal of Jilin University(Medicine Edition)
2020;46(2):309-315
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of lipopolysaccharide (LPS) on the expressions of epithelial-mesenchymal transition (EMT) markers and fi-catenin in the breast cancer MDA-MB-231 cells, and to clarify its possible mechanism. Methods: The breast cancer MDA-MB-231 cells were divided into control group and different concentrations (5, 10, 20, and 40 mg • L _ 1 ) of LPS groups. Inverted microscope was used to observe the morphology of MDA-MB-231 cells in various groups. Immunofluorescence test was used to detect the β-catenin expression and location in the MDA-MB-231 cells in various groups. Real-time quantitative PCR (RT-qPCR) and Western blotting methods were used to detect the expression levels of the E M T markers E-cadherin, Vimentin and β-catenin mRNA and proteins in the MDA-MB-231 cells in various groups. Results: The morphology of MDA-MB-231 cells in control group was epithelial phenotype, and the morphology of MDA-MB-231 cells in different concentrations of LPS groups were the phenotype of mesenchymal cells. The results of immunofluorescence staining showed that the expression of β-catenin was mainly located in the nucleus. Compared with control group, the expression levels of Vimentin and β-catenin mRNA and proteins in the MDA-MB-231 cells in different concentrations of LPS groups were increased (P < 0. 05 or P < 0. 01), especially in 20 mg • L _ 1 LPS group. Compared with control group, the expression levels of E-cadherin mRNA and proteins in the MDA-MB-231 cells in different concentrations of LPS groups were decreased (P < 0. 05 or P < 0. 01), especially in 20 mg • L _ 1 LPS group. Conclusion: LPS could promote the E M T, invasion and metastasis of the breast cancer MDA-MB-231 cells by down-regulating the E-cadherin expression and up-regulating the Vimentin expression, and its mechanism may be related to Wnt/ fj-catenin signaling pathway.
