Effect of microRNA-9-5p targeting MEF2C on biological behaviors of alveolar rhabdomyosarcoma cells
10.13481/j.1671-587x.20200309
- Author:
Hao SHANG
1
Author Information
1. Department of Pathology, School of Medical Sciences, Shihezi University
- Publication Type:Journal Article
- Keywords:
Alveolar rhabdomyosarcoma;
Apoptosis;
Cell proliferation;
Mir-9-5p;
Myocyte enhancer 2c
- From:
Journal of Jilin University(Medicine Edition)
2020;46(3):482-491
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of microRNA-9-5p (miR-9-5p) targeting the myocyte enhancer factor 2C (MKF2C) on the biologicals behaviors of the alveolar rhabdomyosarcoma (ARMS) cells, and to provide the basis for the molecular diagnosis and targeted therapy of ARMS. Methods: The expression levels of miR-9-5p and MKF2C mRNA in ARMS tissue and cells were detected by qRT-PCR method' the proliferation rate of cells was detected by CCK-8 method, the apoptotic rate was detected by flow cytometry, the numbers of invasion and migration cells were detected by Transwell chamber assay, the luciferase activity in 293T cells was detected by double luciferase reporter gene, and the expression level of MEF2C protein in the cells was detected by Western blotting method. Results: The expression levels of miR-9-5p in ARMS tissue and cells were higher than those in normal skeletal muscle tissue and HSKMC cells ( P
