A simple way to improve fibroblast seeding efficiency and uniformity in constructing 3-D dermal equivalent
- Author:
Juan ZHOU
1
Author Information
1. Institute of Biomedical Engineering
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(1):101-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore a simple way to improve seeding efficiency and uniformity in constructing 3-D dermal equivalent, in order to effectively construct tissue engineered skin. Methods: Human dermal fibroblasts were added onto collagen sponge scaffolds and cultured in the following 3 ways: (1) oscillating seeding and oscillating culture (OO), (2) oscillating seeding and static culture (OS), and (3) static seeding and static culture (SS). Samples were obtained and subjected to light microscopy, scanning electron microscopy (SEM) and quantitative cell number assay at planed times. Results: Oscillating seeding achieved more uniform spatial cell distribution within scaffolds as compared to static seeding. Moreover, fibroblast seeding efficiency was significantly higher in oscillating conditions ([56±5]%) than in static conditions([31=4]%, P<0.01). Cells from OO samples died due to their breaking away from the dermal frame and were discarded. Cells from OS samples and SS samples remained a normal extension. The cell ingrowth and the cell number were both higher for OS samples than that for SS throughout the culture. Conclusion: These results suggest that, as compared to conventional static techniques, oscillating seeding of dermal fibroblasts on 3-D scaffolds can obviously improve seeding efficiency and uniformity.
