Mitochondria gene expression in human liver cancer cell line SMMC-7721
- Author:
Jie WANG
1
Author Information
1. Department of Biochemistry and Molecular Biology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(1):46-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the mitochondria gene expression in human liver cancer cell line SMMC-7721. Methods: Four pairs of primers for multiple PCR were designed according to the sequence of human mtDNA with primer premier 5.0 biosoft. The primers were then used to amplify D-loop region and the gene ND6, ATPase8-ATPase6 and 16sRNA. The expression of D-loop region and the 3 genes of mtDNA in SMMC-7721 was measured by RT-PCR, and the results were compared with those in normal human liver cells (LO2). Results: Compared with those in LO2 cells, the expression of the D-loop region, ND6, ATPase8-ATPase6 and 16sRNA subunits in SMMC-7721 cells was increased, and the expression of l6sRNA subunit remained unchanged. Meanwhile, there was difference in the transcription of H-strand and L-strand of a certain gene. Conclusion: The mtDNA-encoded polypeptides are enzyme complex subunits of the oxidative phosphorylation compound, and their aberrant expression harms the oxidative phosphorylation capacity of cells and leads to decreased synthesis of ATP, which may be one of the reasons for glycolysis of SMMC-7721 cells.
