Effect of high zinc concentrations on contents of iron and zinc and expression of their regulating mRNA in Caco-2 cells
- Author:
Yi ZHANG
1
Author Information
1. Department of Military Hygiene
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(1):41-45
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of high zinc concentrations on contents of iron and zinc and expression of their regulating mRNA in Caco-2 cells. Methods: Caco-2 cells were used as model of human small intestinal enterocytes and were treated for 24 h with 4, 50,100 and 200 μmol/L of zinc. The zinc and iron contents were determined by atom absorption spectrophotography and the expression of DMT1, IREG1, ZnT1 and hZIP4 mRNA were determined by RT-PCR (the level of GAPDH was taken as internal control). Results: The zinc content in Caco-2 cells was increased 24 h after treatment with zinc, and reached the peak when exposed to 50 μmol/L zinc. The iron content decreased with the increase of zinc concentration. Zinc supplement increased the expressions of DMT1, IREG1 and ZnT1 mRNA but decreased the expression of hZIP4 mRNA. Conclusion: It is suggested that zinc supplement can increase the contents of zinc but decrease the contents of iron in Caco-2 cells. There may be interactions between iron and zinc in the intestinal lumen.
