Gene expression profile during early period of smooth muscle cell proliferation in macroangiopathy in diabetic rats with spontaneous hypertension
- Author:
Yong YE
1
Author Information
1. Department of Human Anatomy
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(1):36-40
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the gene expression profile during early period of smooth muscle cell(SMC) proliferation in macroangiopathy in diabetic rats with spontaneous hypertension. Methods: Spontaneously hypertensive rats (SHR) were taken as control and a hypertensive diabetic rat model was established by streptozotocin (STZ) injection in SHR. The total RNAs were obtained from intima-media of thoracic aortas in 1-week, 2-week and 4-week hypertensive diabetic rats separately. The mRNAs from SHR and hypertensive diabetic rats were reversely transcribed into the cDNAs. The 2 probes were then hybridized to the cDNA microarray containing 4 096 full length genes from rats. After high-stringent washing, the cDNA microarray was scanned and analyzed by computer image analysis. Bioinformatic analysis of altered gene expression was performed to find out the possible genes related to diabetic macroangiopathy. Results: A total of 321 genes were screened out in 1-week hypertensive diabetic rats, with 160 genes down-regulated (ratio<0.5-fold) and 161 up-regulated (ratio>2-fold); 414 genes were screened out in 2-week hypertensive diabetic rats with 128 down-regulated and 176 up-regulated; and 403 genes were screened out in 4-week hypertensive diabetic rats with 202 down-regulated and 201 upregulated. The effective genes were divided into 8 groups by cluster analysis. Thirteen known genes were up-regulated in all 3 kinds of hypertensive diabetic rats, among which CD74, Irf1, GAP43 and CD36 had a steady increase and were related to cell proliferation. Furthermore, CYP2E1 gene was obviously up-regulated (ratio for the 1-week rats was 34. 54,2-week was 24. 82, and 3-week was 33.57). Conclusion: The gene expression profile based on cDNA micrioarray can be used for screening genes related with diabetic macroangiopathy. It is found that CYP2E1 might be an important gene responsible for proliferation of SMC in diabetic macroangiopathy.
