Extraction of cuttlebone polysaccharides and purification of their active component CPS-1
- Author:
Jiang-Zhou WEI
1
Author Information
1. Department of Biochemistry and Molecular Biology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(1):17-21
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To extract the active polysaccharides from traditional Chinese herb-cuttlebone and to purify cuttlebone polysacchride salts 1(CPS-1), an active component of crude polysaccharides, so as to obtain refined natural active polysaccharides. Methods: Hot-water extraction method was optimized by orthogonal designing and was used to extract crude polysacchaides from cuttlebone. The total sugar contents of crude polysaccharides were determined. DEAE Sepharose. F. F column and Sepharose CL-6B column were applied to separate CPS-1 from the crude polysaccharides. The active components were determined by animal experiments and Sephacryl S-300 column was used to further purification of CPS-1. HPLC was used to determine the purify of refined CPS-1 and the molecular weight of CPS-1 was determined by polysaccharides standard curve. Results: Crude polysaccharides were successfully extracted from cuttlebone. After purification with DEAE Sepharose F. F column, Sepharose CL-6B column and Sephacryl S-300 column and activity study, refined CPS-1 with average molecular weight (1 × 106 was obtained, and the sugar content reached 93.6%. Conclusion: Different extraction conditions have different extraction results for crude polysaccharides and different separation materials can separate the mixed polysaccharides by their molecular weights or charge characteristics. CPS-1 is a natural active polysaccharide extracted from cuttlebone.
