Preparation, identification and immunological activities of polysaccharides extracted from Mytilus coruscus
- Author:
Jun WANG
1
Author Information
1. Department of Biochemistry and Molecular Biology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(1):12-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To isolate and purify active polysaccharides from Mytilus coruscus (MPs) and to determine their purities and monosaccharide components. Methods: The homogenization of Mytilus coruscus was extracted by boiling water, followed by protein removal (Sevage method), alcohol fractional precipitation, chromatography with DEAE-cellulose 52, Sephacryl S-200 column and HPLC. The sugar concentrations in MPs were determined by λmax of full wave-length scan and sulphuric acid-phenol colorimetry. IR chromatogram, UV absorption spectrum, staining-combined colorimetry and HPLC were separately applied to determine the homogeneity of the obtained MPS; GC-MS was used to analyze their monosaccharide components. The immunological activities of purified MPs were tested. Results: Eight components, MPs-A1/A2/B1/B2/B3/C1/C2/C3, were extracted from Mytilus coruscus. HNMR indicated that MPs-B1, with a molecular weight of 3 510 000, was an α-hexapyranose, with glucose and galactose (1 : 1) as its major components. Immunological tests showed that MPs-B1 promoted the activities of NK cells, peritoneal marcophages and plaque forming cells. Conclusion: With modified preparation process, the MPs in this paper are obviously different from those obtained from other similar studies in physiochemical properties, sugar contents and monosaccharide components. MPs-B1 has a positive regulatory immunological function.
