Effect of ulinastatin on expression of inflammatory cytokines during perioperative period of surgical correction of adolescent idiopathic scoliosis
- Author:
Wei ZHU
1
Author Information
1. Department of Anesthesiology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(2):203-206
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of ulinastatin(UTI) on the changes of IL-6, TNF-α, IL-10 and IL-4 expression during perioperative period of surgical correction of adolescent idiopathic scoliosis(AIS). Methods: Twenty patients with AIS I-II scheduled to receive surgical correction of spinal deformities were equally randomized into 2 groups-UTI group and control group. Patients in UTI group received intravenous infusion of 10 000 U/kg UTI with 250 ml normal saline just before operation and every 4 h thereafter if necessary; patients in control group received same amount of normal saline. ECG, CVP, SpO2 and PETCO2 were continously monitored during operation in both group. Mean arteral pressure (MAP) was maintained at (60±5) mmHg (1 mmHg=0.133 kPa). Venous blood samples were collected immediately before induction of anaesthesia (T1), 10 min after induction (T2), 1 h after UTI administration (T3), 30 min after extubation (T4) and 24 h postoperatively (T5). The plasma levels of IL-6, TNF-α, IL-10 and IL-4 were measured by enzyme-linked immunosorbent assay (ELISA), and their mRNA expression was assayed by real-time quantitative reverse transcription polymerase chain reaction. Results: At T3, T4 and T5, plasma levels of IL-6 and TNF-α and their mRNA copies in control group were significantly higher than that at T1 (P<0.05 or P<0.01) and those in UTI group(P<0.05 or P<0.01); while plasma IL-6 and TNF-α and their mRNA copies in UTI group had no difference compared with those at T1, but were significantly lower than those of control group (P<0.05 or P<0.01). At T4 and T5, plasma IL-10 and its mRNA copies had a significant increase compared with those at T1 in control group (P<0.05). At T4 and T5, plasma IL-10 and its mRNA copies had a significant increase compared with those at T1 in UTI group (P<0.01), and were higher than that of control group (P<0.01). The plasma IL-4 and its mRNA copies had no significant change in both groups on each point time. Conclusion: UTI can inhibit the release of IL-6, TN F-α and their mRNA copies in perioperation period of surgical correction of AIS, and it may also promote expression of IL-10 protein and IL-10 mRNA.
