Isoflurane ameliorates oxygen supply-consumption balance after anoxia and reoxygenation in isolated rat liver
- Author:
Quan LI
1
Author Information
1. Department of Anesthesiology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(2):200-202
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the protective effects of isoflurane on liver flow and oxygen consumption after anoxia and reoxygenation in isolated rat liver. Methods: The perfusion model of isolated rat liver was established. Rat livers were harvested and were perfused with modified Krebs' buffer, which was saturated with 95% O2, 5% CO2 and contained 10 mmol/L glucose (for nutrition) and 1% albumin (for osmatic pressure). The perfusion pressure was kept at 1.2 kPa for oxygenation and 0.2 kPa for anoxia. Meanwhile, pH value and temperature were properly maintained and oxygen pressure and liver flow were measured. Different concentrations of isoflurane were fed into artificial lung with mixed air. Results: Is oflurane had no obvious influence on liver flow under basal condition,but improved liver flow after liver was exposed to anoxia and reoxygenation. It also decreased liver oxygen consumption under basal condition and improved liver oxygen consumption after liver was exposed to anoxia and reoxygenation. Conclusion: Isoflurane can improve oxygen supply-consumption balance after anoxia and reoxygenation in isolated rat liver.
