Protective effects of self-designed Shanghai multi-organ preservation solution on kidney cortex mitochondria function during cold storage
- Author:
Qiang NIU
1
Author Information
1. Organ Transplantation Institute of PLA
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(2):196-199
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the protective effect of self-designed Shanghai multi-organ preservation solution(SMO) on mitochondria function in canine kidney cortex during cold storage. Methods: Canine kidneys were perfused and stored with cold (0-4 °C) SMO, HC-A and UW solution (the later 2 as control) separately. Ultrastructure alteration of mitochondria was observed by electron microscopy. Mitochondria were isolated from cortex homogenization by differential centrifugation and their respiratory function was measured polarographically with Clark oxygen electrode. The four-state curve of mitochondria respiration was drawn and the state III and IV oxygen consumption rate, the respiration control proportion(RCR) and phosphatase1/oxygen ratio(P/O) were calculated. Results: We found that the mitochondria swelling and membrane disruption in HC-A group were more severe than those in SMO and UW group. The rate of oxygen consumption in state III, RCR and P/O were obviously decreased in all groups with the prolongation of cold storage time. The rate of oxygen consumption in state III in HC-A group was lower compared to that in SMO group during 1-3 d cold storage(P<0.05). The rate of oxygen consumption in state IV was slightly increased during the cold storage, but there was no statistical difference between HC-A and SMO group. RCR and P/O in SMO group were statistically higher than those in HC-A group (P<0.05) and were similar to those of UW group. Conclusion: SMO solution has similar protective effect on cortex mitochondria function impairment induced by hypothermia and ischemia during cold storage as UW solution, and the effect is more potent than that of HC-A solution.
