Antioxidative effects of rosiglitazone on rat kidney with unilateral ureteral obstruction
- Author:
Qin LIN
1
Author Information
1. Department of Nephrology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(2):192-195
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the antioxidative effect of rosiglitazone(RSG), a new insulin sensitizer, on kidney of rats with unilateral ureteral obstruction (UUO). Methods: Male adult Sprague-Dawley rats were randomly divided into 3 groups: Sham-operated group (n=6), UUO model group (UUO model was established by ligating the left ureter, n=18), and UUO+RSG group (UUO model rats were treated with RSG, n=18). The latter 2 groups were divided into subgroups according to the times (3 d, 7 d and 14 d) after intervention (n=6). Malondialdehyde (MDA), catalase (CAT) and superoxide dismutase (SOD) contents in the homogenized lysate of obstructive renal cortex were determined. Results: Compared with those in Sham-operated group, MDA contents in UUO group increased by 8.9 (P=0.000), 3.5 (P=0.000) and 0.7 times on d 3, d 7 and d 14, respectively, while the contents of CAT and SOD decreased dramatically, with CAT contents reduced by 66.0%, 72.6% and 83.8%, respectively (P=0.000) and SOD contents reduced by 42.0%, 50.2% and 67.7%, respectively (P<0.005). Compared with those in UUO model group, MDA contents in UUO+RSG group decreased by 80.0% and 65.3% on d 3 and d 7 (P=0.000), respectively; no significant difference was found between the 2 contents on d 14; CAT contents were increased by 96.2%, 92.1% and 102.9%, respectively (P<0.005) and SOD content were increased by 33.3%, 35.1% and 55.4% (P<0.005), respectively. Conclusion: Rosiglit azone can suppress oxidative stress in rats with UUO by reducing lipid peroxidation production and improving antioxidative enzyme contents.
