Overexpression of glucocorticoid receptor beta affects regulatory effect of glucocorticoids on proliferation and differentiation of human osteosarcoma cells
- Author:
Yun ZHOU
1
Author Information
1. Department of Endocrinology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(2):186-188
- CountryChina
- Language:Chinese
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Abstract:
Objective: To study the effect of glucocorticoid receptor beta overexpression on the regulatory effect of glucocorticoid on proliferation and differentiation of human osteosarcoma cell and to investigate the biological significance of glucocorticoid receptor beta. Methods: Glucocorticoid receptor beta was stably transfected into human osteosarcoma cells and the stable transfectants were treated with different concentrations of dexamethasone (10-10-10-6 mol/L). Then cell proliferation was determined by MTT method and viable cell counting; alkaline phosphatase was detected by standard hydroxybenzene colorimetry. Results and conclusion: In stable transfectants overexpres sing glucocorticoid receptor beta, glucocorticoid-induced cell proliferation repression and cell differentiation are inhibited, indicating that glucocorticoid receptor beta may be an endogenous inhibitor of glucocorticoid receptor alpha.
