In vitro effects of propofol on apoptosis and Bax expression induced by TNF-α in mouse spinal cord neurons
- Author:
Jian-Hua XIA
1
Author Information
1. Department of Anesthesiology
- Publication Type:Journal Article
- From:
Academic Journal of Second Military Medical University
2006;27(2):169-172
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the in vitro effects of propofol on apoptosis and Bax expression induced by TNF-α in mouse spinal cord neurons. Methods: Spinal cord neurons were isolated from fetal mice and cultured in neurobasal medium with B27 supplement. On the 7th day of culture, neurons were randomly divided into 6 groups: control group, propofol (50 μmol/L) group, TNF-α group, propofol(25 μmol/L) with TNF-α group, propofol(50 μmol/L) with TNF-α group and propofol(100 μmol/L) with TNF-α group. Propofol with different concentrations was incubated with cultured cells for 30 min, then TNF-α was added with the final concentration of 2 000 U/ml for another 24 h incubation. Apoptosis was detected by PI/Hoechst33342 double staining technique and fluorescence microscopy. Bax expression was determined by immunocytochemical technique. Results: The apoptosis rate and expression of Bax in TNF-α group were both increased compared with those in control group ([21.8±1.1]% vs [2.8±0.8]%,P<0.01; [0.251±0.016] vs [0.141±0.015],P<0.01). The apoptosis rates and expression of Bax in 3 propofol groups(25, 50, 100 μmol/L) were ([16.2±1.2]%, [15.3±0.6]% and [12.2±0.8]%) and ([0.198±0.011],[0.188±0.012] and [0.167±0.014]), respectively, and they were all significantly decreased compared with those in TNF-α group ([21.8±1.1]% and [0.251±0.0163], all P<0.05). Moreover, a dose-dependent manner was observed in the above changes. Conclusion: Propofol within clinical concentration ranges may inhibit the apoptosis of spinal cord neurons induced by TNF-α through modulating the expression of Bax.
